Coding

Part:BBa_K916004:Design

Designed by: Joseph Elsherbini   Group: iGEM12_Georgia_Tech   (2012-10-02)

Leucine Zipper CGFP translational fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 292


Design Notes

This part is compatible in its current form with most biobrick assembly methods. There is no promoter or RBS with this part, so one can select the desired promoter and the strength of the RBS from other parts in the registry.


Source

The CGFP fragment came from a plasmid (pMRBAD-z-NGFP) provided by Dr. Lynne Regan at Yale University.

References

Wilson CGM, Magliery TJ, Regan L. 2004. Detecting protein-protein interactions with GFP-fragment reassembly. Nat Methods 1: 255. [http://www.yale.edu/reganlab/pdfs/WilsonRegan2004NatMethods.pdf PDF.]